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验证数据展示

  • WB analysis of human kidney using 16046-1-AP (same clone as 16046-1-PBS)

    WB analysis of human kidney using 16046-1-AP (same clone as 16046-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

    IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

    IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 16046-1-AP (same clone as 16046-1-PBS)

    IF Staining of HEK-293 using 16046-1-AP (same clone as 16046-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16046-1-AP (ALG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

ALG5 Polyclonal antibody, PBS Only

ALG5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16046-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16046-1-PBS targets ALG5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8990

Product name: Recombinant human ALG5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 34-324 aa of BC012531

Sequence: MPALHRHEEEKFFLNAKGQKETLPSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSKDQTSKVAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDVEKLEKGLNDLQPWPNQMAIACGSRAHLEKESIAQRSYFRTLLMYGFHFLVWFLCVKGIRDTQCGFKLFTREAASRTFSSLHVERWAFDVELLYIAQFFKIPIAEIAVNWTEIEGSKLVPFWSWLQMGKDLLFIRLRYLTGAWRLEQTRKMN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
别名
计算分子量 324 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC012531
基因名称 ALG5
Gene ID (NCBI) 29880
RRIDAB_2273966
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y673
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.

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