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验证数据展示

  • WB analysis of human kidney using 16046-1-AP

    WB analysis of human kidney using 16046-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 16046-1-AP

    IHC staining of mouse liver using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 16046-1-AP

    IHC staining of mouse liver using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 16046-1-AP

    IF Staining of HEK-293 using 16046-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16046-1-AP (ALG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ALG5 Polyclonal antibody

ALG5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16046-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

HSPC149, EC:2.4.1.117, DolP-glucosyltransferase, Dolichyl-phosphate beta-glucosyltransferase, Asparagine-linked glycosylation protein 5 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue, mouse liver tissue
Positive IHC detected inmouse liver tissue, human liver cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16046-1-AP targets ALG5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 ALG5 fusion protein Ag8990 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
别名 HSPC149, EC:2.4.1.117, DolP-glucosyltransferase, Dolichyl-phosphate beta-glucosyltransferase, Asparagine-linked glycosylation protein 5 homolog
计算分子量 324 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC012531
基因名称 ALG5
Gene ID (NCBI) 29880
RRIDAB_2273966
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y673
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.

实验方案

Product Specific Protocols
WB protocol for ALG5 antibody 16046-1-APDownload protocol
IHC protocol for ALG5 antibody 16046-1-APDownload protocol
IF protocol for ALG5 antibody 16046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Cell Mol Life Sci

ALG5 downregulation inhibits osteogenesis and promotes adipogenesis by regulating the N-glycosylation of SLC6A9 in osteoporosis

Authors - Quanfeng Li
  • KD Validated
View Article