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验证数据展示

  • IHC staining of mouse kidney using 16207-1-AP (same clone as 16207-1-PBS)

    IHC staining of mouse kidney using 16207-1-AP (same clone as 16207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16207-1-AP (ALG14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16207-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 16207-1-AP (same clone as 16207-1-PBS)

    IF Staining of A431 using 16207-1-AP (same clone as 16207-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ALG14 antibody (16207-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16207-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 16207-1-AP (same clone as 16207-1-PBS)

    IHC staining of mouse kidney using 16207-1-AP (same clone as 16207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16207-1-AP (ALG14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16207-1-PBS in a different storage buffer formulation.

ALG14 Polyclonal antibody, PBS Only

ALG14 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 16207-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

Asparagine-linked glycosylation 14 homolog, UDP-N-acetylglucosamine transferase subunit ALG14

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16207-1-PBS targets ALG14 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9178

Product name: Recombinant human ALG14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-216 aa of BC011706

Sequence: RSMDVTPRESLSILVVAGSGGHTTEILRLLGSLSNAYSPRHYVIADTDEMSANKINSFELDRADRDPSNMYTKYYIHRIPRSREVQQSWPSTVFTTLHSMWLSFPLIHRVKPDLVLCNGPGTCVPICVSALLLGILGIKKVIIVYVESICRVETLSMSGKILFHLSDYFIVQWPALKEKYPKSVYLGRIV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asparagine-linked glycosylation 14 homolog (S. cerevisiae)
别名 Asparagine-linked glycosylation 14 homolog, UDP-N-acetylglucosamine transferase subunit ALG14
计算分子量 216 aa, 24 kDa
GenBank蛋白编号BC011706
基因名称 ALG14
Gene ID (NCBI) 199857
RRIDAB_3741878
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96F25
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALG14 (UDP-N-Acetylglucosaminyltransferase Subunit ALG14 Homolog) is a protein involved in the process of N-linked glycosylation, which is essential for protein folding and stability. ALG14 forms a heterodimeric UDP-N-acetylglucosamine transferase (GnTase) with ALG13, which catalyzes a key step in the synthesis of the lipid-linked oligosaccharide (LLO) precursor during N-linked glycosylation (PMID: 36200043).

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