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验证数据展示

  • IHC staining of mouse kidney using 16207-1-AP

    IHC staining of mouse kidney using 16207-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16207-1-AP (ALG14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 16207-1-AP

    IF Staining of A431 using 16207-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ALG14 antibody (16207-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse kidney using 16207-1-AP

    IHC staining of mouse kidney using 16207-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16207-1-AP (ALG14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ALG14 Polyclonal antibody

ALG14 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 16207-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

Asparagine-linked glycosylation 14 homolog, UDP-N-acetylglucosamine transferase subunit ALG14

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16207-1-AP targets ALG14 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9178

Product name: Recombinant human ALG14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-216 aa of BC011706

Sequence: RSMDVTPRESLSILVVAGSGGHTTEILRLLGSLSNAYSPRHYVIADTDEMSANKINSFELDRADRDPSNMYTKYYIHRIPRSREVQQSWPSTVFTTLHSMWLSFPLIHRVKPDLVLCNGPGTCVPICVSALLLGILGIKKVIIVYVESICRVETLSMSGKILFHLSDYFIVQWPALKEKYPKSVYLGRIV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asparagine-linked glycosylation 14 homolog (S. cerevisiae)
别名 Asparagine-linked glycosylation 14 homolog, UDP-N-acetylglucosamine transferase subunit ALG14
计算分子量 216 aa, 24 kDa
GenBank蛋白编号BC011706
基因名称 ALG14
Gene ID (NCBI) 199857
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96F25
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALG14 (UDP-N-Acetylglucosaminyltransferase Subunit ALG14 Homolog) is a protein involved in the process of N-linked glycosylation, which is essential for protein folding and stability. ALG14 forms a heterodimeric UDP-N-acetylglucosamine transferase (GnTase) with ALG13, which catalyzes a key step in the synthesis of the lipid-linked oligosaccharide (LLO) precursor during N-linked glycosylation (PMID: 36200043).

实验方案

Product Specific Protocols
IF protocol for ALG14 antibody 16207-1-APDownload protocol
IHC protocol for ALG14 antibody 16207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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