验证数据展示

WB analysis of HeLa using 11217-1-AP
WB result of ALDOA antibody (11217-1-AP; 1:240000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HeLa cells.
WB analysis using 11217-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 11217-1-AP
IP result of anti-ALDOA (IP:11217-1-AP, 3ug; Detection:11217-1-AP 1:1000) with HepG2 cells lysate 6000ug.
WB analysis using 11217-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11217-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 11217-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ALDOA antibody included
IHC staining of human colon cancer using 11217-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ALDOA antibody included
IF Staining of HeLa using 11217-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO验证

ALDOA Polyclonal antibody

ALDOA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11217-1-AP

产品说明书

引用文献(74)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

别名

Muscle-type aldolase, Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (74)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, HeLa cells, A549 cells, Jurkat cells, mouse liver tissue, rat liver tissue, HepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 7 publications below
WB	See 65 publications below
IHC	See 11 publications below
IF	See 10 publications below
IP	See 6 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

产品信息

11217-1-AP targets ALDOA in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, RIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, sheep
免疫原	CatNo: Ag1726 Product name: Recombinant human ALDOA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-364 aa of BC016800 Sequence: PYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	aldolase A, fructose-bisphosphate
别名	Muscle-type aldolase, Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A
计算分子量	39 kDa
观测分子量	35-40 kDa
GenBank蛋白编号	BC016800
基因名称	ALDOA
Gene ID (NCBI)	226
RRID	AB_2224626
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P04075
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

实验方案

Product Specific Protocols
IF protocol for ALDOA antibody 11217-1-AP	Download protocol
IHC protocol for ALDOA antibody 11217-1-AP	Download protocol
IP protocol for ALDOA antibody 11217-1-AP	Download protocol
WB protocol for ALDOA antibody 11217-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1. Authors - Ling Li View Article
mouse	WB	Nat Neurosci Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex. Authors - Xiaoxiang Dong View Article
mouse	WB	Dev Cell Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development. Authors - Vinay Bulusu View Article
human,mouse	WB	Int J Biol Sci Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway Authors - Yunfeng Guan View Article
human	WB	Cell Death Dis The ubiquitin-like protein UBTD1 promotes colorectal cancer progression by stabilizing c-Myc to upregulate glycolysis Authors - Liqin Zhao View Article
human	IF	Mol Ther Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes. Authors - Junyi Hu View Article

验证数据展示

WB analysis of HeLa using 11217-1-AP

WB analysis using 11217-1-AP

IP experiment of HepG2 using 11217-1-AP

WB analysis using 11217-1-AP

WB analysis using 11217-1-AP

IHC staining of human colon cancer using 11217-1-AP

IHC staining of human colon cancer using 11217-1-AP

IF Staining of HeLa using 11217-1-AP

ALDOA Polyclonal antibody

ALDOA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.

Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway

The ubiquitin-like protein UBTD1 promotes colorectal cancer progression by stabilizing c-Myc to upregulate glycolysis

Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.