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KD/KO Validated

ALDOA Polyclonal antibody

ALDOA Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat No : 11217-1-AP

Print datasheet

Synonyms

ALDA, ALDOA, Aldolase A, Lung cancer antigen NY LU 1, Muscle type aldolase

验证数据展示

IF Staining of HeLa using 11217-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis of HeLa using 11217-1-AP
WB result of ALDOA antibody (11217-1-AP; 1:240000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HeLa cells.
WB analysis using 11217-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 11217-1-AP
IP result of anti-ALDOA (IP:11217-1-AP, 3ug; Detection:11217-1-AP 1:1000) with HepG2 cells lysate 6000ug.
WB analysis using 11217-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11217-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 11217-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human colon cancer using 11217-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ALDOA antibody included
IHC staining of human colon cancer using 11217-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ALDOA antibody included
FC experiment of HeLa using 11217-1-AP
1X10^6 HeLa cells were stained with 0.2ug ALDOA antibody (11217-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 11217-1-AP

Size

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经过测试的应用

Positive WB detected in	HEK-293 cells, A549 cells, HeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells
Positive FC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 5 publications below
WB	See 41 publications below
IHC	See 6 publications below
IF	See 8 publications below
IP	See 3 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

产品信息

11217-1-AP targets ALDOA in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications	CoIP, IF, IHC, IP, RIP, WB
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, sheep, pig
Immunogen	ALDOA fusion protein Ag1726 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	aldolase A, fructose-bisphosphate
Synonyms	ALDA, ALDOA, Aldolase A, Lung cancer antigen NY LU 1, Muscle type aldolase
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	35-40 kDa
GenBank Accession Number	BC016800
Gene Symbol	ALDOA
Gene ID (NCBI)	226
RRID	AB_2224626
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04075
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

实验方案

Product Specific Protocols
WB protocol for ALDOA antibody 11217-1-AP	Download protocol
IHC protocol for ALDOA antibody 11217-1-AP	Download protocol
IF protocol for ALDOA antibody 11217-1-AP	Download protocol
IP protocol for ALDOA antibody 11217-1-AP	Download protocol
FC protocol for ALDOA antibody 11217-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1. Authors - Ling Li View Article
mouse	WB	Nat Neurosci Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex. Authors - Xiaoxiang Dong View Article
mouse	WB	Dev Cell Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development. Authors - Vinay Bulusu View Article
human,mouse	WB	Int J Biol Sci Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway Authors - Yunfeng Guan View Article
human	IF	Mol Ther Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes. Authors - Junyi Hu View Article
mouse	WB	eLife Glycolytic flux-signaling controls mouse embryo mesoderm development Authors - Hidenobu Miyazawa View Article

ALDOA Polyclonal antibody

ALDOA Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

验证数据展示

IF Staining of HeLa using 11217-1-AP

WB analysis of HeLa using 11217-1-AP

WB analysis using 11217-1-AP

IP experiment of HepG2 using 11217-1-AP

WB analysis using 11217-1-AP

WB analysis using 11217-1-AP

IF Staining of HeLa using 11217-1-AP

IHC staining of human colon cancer using 11217-1-AP

IHC staining of human colon cancer using 11217-1-AP

FC experiment of HeLa using 11217-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.

Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway

Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.

Glycolytic flux-signaling controls mouse embryo mesoderm development