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ALDH8A1 Polyclonal antibody

ALDH8A1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21602-1-AP

Print datasheet

Synonyms

Aldehyde dehydrogenase 12, ALDH12, ALDH8A1

验证数据展示

  • WB analysis using 21602-1-AP

    WB analysis using 21602-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 21602-1-AP

    IHC staining of human hepatocirrhosis using 21602-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 21602-1-AP

    IHC staining of human hepatocirrhosis using 21602-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 21602-1-AP

    IF Staining of HepG2 using 21602-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21602-1-AP is ALDH8A1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen ALDH8A1 fusion protein Ag16214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aldehyde dehydrogenase 8 family, member A1
Synonyms Aldehyde dehydrogenase 12, ALDH12, ALDH8A1
Calculated Molecular Weight 487 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC113862
Gene Symbol ALDH8A1
Gene ID (NCBI) 64577
RRIDAB_10733887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2A2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALDH8A1 antibody 21602-1-APDownload protocol
IHC protocol for ALDH8A1 antibody 21602-1-APDownload protocol
IF protocol for ALDH8A1 antibody 21602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols