验证数据展示
经过测试的应用
Positive WB detected in | rat liver tissue, mouse heart tissue, mouse kidney tissue, HEK-293 cells, rat kidney tissue, mouse liver tissue |
Positive IHC detected in | human liver tissue, human kidney tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:3000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
产品信息
20452-1-AP targets ALDH6A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | ALDH6A1 fusion protein Ag13879 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | aldehyde dehydrogenase 6 family, member A1 |
别名 | ALDH6A1, MMSADHA, MMSDH |
计算分子量 | 535 aa, 58 kDa |
观测分子量 | 58 kDa |
GenBank蛋白编号 | BC004909 |
基因名称 | ALDH6A1 |
Gene ID (NCBI) | 4329 |
RRID | AB_10697664 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q02252 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ALDH6A1 antibody 20452-1-AP | Download protocol |
IHC protocol for ALDH6A1 antibody 20452-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancers (Basel) RNA Sequencing of Collecting Duct Renal Cell Carcinoma Suggests an Interaction between miRNA and Target Genes and a Predominance of Deregulated Solute Carrier Genes. | ||
Front Neurol Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein. | ||
Theranostics DNMT1 mediates the disturbed flow-induced endothelial to mesenchymal transition through disrupting β-alanine and carnosine homeostasis |