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验证数据展示

  • FC experiment of human PBMCs using 98645-2-RR

    FC experiment of human PBMCs using 98645-2-RR

    1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Rabbit RecAb (98645-2-RR, Clone: 251789D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of human PBMCs using 98645-2-RR

    FC experiment of human PBMCs using 98645-2-RR

    1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Rabbit RecAb (98645-2-RR, Clone: 251789D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Rabbit RecAb (98645-2-RR, Clone:251789D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

  • Affinity of 98645-2-RR

    Affinity of 98645-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98645-2-RR against Human ALCAM were performed. The affinity constant is below 1 pM.

Anti-Human ALCAM Rabbit Recombinant Antibody

ALCAM Uni-rAb® Recombinant Antibody for FC
Cat No. 98645-2-RR

产品说明书

CloneNo. 251789D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD166, MEMD

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
PerCP-Cyanine5.5
规格价格库存

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经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98645-2-RR targets ALCAM in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg5038

Product name: Recombinant Human ALCAM protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 28-527 aa of BC137097

Sequence: WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activated leukocyte cell adhesion molecule
别名 CD166, MEMD
计算分子量 583 aa, 65 kDa
GenBank蛋白编号BC137097
基因名称 ALCAM
Gene ID (NCBI) 214
RRIDAB_3746242
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13740
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

实验方案

Product Specific Protocols
FC protocol for ALCAM antibody 98645-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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