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验证数据展示

  • WB analysis using 86811-1-RR (same clone as 86811-1-PBS)

    WB analysis using 86811-1-RR (same clone as 86811-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86811-1-RR (ALCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86811-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02780-1

    Sandwich ELISA sample test of MP02780-1

    The mean ALCAM concentration was determined to be 274.2 ng/mL in HT-1080 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA sample test of MP02780-1

    Sandwich ELISA sample test of MP02780-1

    Plasma of six individual healthy human donors was measured. The ALCAM concentration of detected samples was determined to be 540.4 ng/mL with a range of 347.0-752.3 ng/mL.

  • Sandwich ELISA standard curve of MP02780-1

    Sandwich ELISA standard curve of MP02780-1

    Sandwich ELISA standard curve of MP02780-1, Human ALCAM Recombinant Matched Antibody Pair - PBS only. 86811-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg5038. 86811-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • IF Staining of A549 using 86811-1-RR (same clone as 86811-1-PBS)

    IF Staining of A549 using 86811-1-RR (same clone as 86811-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALCAM antibody (86811-1-RR, Clone: 251789C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86811-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86811-1-RR

    Affinity and Kinetic Characterization of 86811-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86811-1-RR against Human ALCAM were performed. The affinity constant is 21.6 pM.

ALCAM Recombinant monoclonal antibody, PBS Only (Detector)

ALCAM Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86811-1-PBS

产品说明书

CloneNo. 251789C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

CD166, MEMD

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86811-1-PBS targets ALCAM as part of a matched antibody pair:

MP02780-1: 86811-3-PBS capture and 86811-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg5038

Product name: Recombinant Human ALCAM protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 28-527 aa of BC137097

Sequence: WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activated leukocyte cell adhesion molecule
别名 CD166, MEMD
计算分子量 583 aa, 65 kDa
观测分子量100 kDa
GenBank蛋白编号BC137097
基因名称 ALCAM
Gene ID (NCBI) 214
RRIDAB_3745134
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13740
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

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