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验证数据展示

  • IF Staining of hTERT-RPE1 using 65611-1-MR

    IF Staining of hTERT-RPE1 using 65611-1-MR

    Immunofluorescent analysis of un-fixed hTERT-RPE1 cells using ALCAM antibody (65611-1-MR, Clone: 3A6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • FC experiment of human PBMCs using 65611-1-MR

    FC experiment of human PBMCs using 65611-1-MR

    1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Untreated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (black). Cells were not fixed.

Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Antibody

ALCAM Uni-rAbTM Recombinant Antibody for IF/ICC, FC
Cat No. 65611-1-MR

产品说明书

CloneNo. 3A6

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC

别名

MEMD, CD166, 3A6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
Positive FC detected inPHA treated human PBMCs

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65611-1-MR targets ALCAM in IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human
免疫原 Cultured human thymic epithelial cells 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 activated leukocyte cell adhesion molecule
别名 MEMD, CD166, 3A6
计算分子量 105 kDa
GenBank蛋白编号BC057809
基因名称 ALCAM
Gene ID (NCBI) 214
RRIDAB_3670358
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

实验方案

Product Specific Protocols
IF protocol for ALCAM antibody 65611-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols