验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, Raji cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| IF | See 1 publications below |
产品信息
16200-1-AP targets ALAS1 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9126 Product name: Recombinant human ALAS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC011798 Sequence: MESVVRRCPFLSRVPQAFLQKAGKSLLFYAQNCPKMMEVGAKPAPRALSTAAVHYQQIKETPPASEKDKTAKAKVQQTPDGSQQSPDGTQLPSGHPLPATSQGTASKCPFLAAQMNQRGSSVFCKASLELQEDVQEMNAVRKEVAETSAGPSVVSVKTDGGDPSGLLKNFQDIMQKQRPERVSHLLQDNLPKSVSTFQYDRFFEKKIDEKKNDHTYRVFKTVNRRAHIFPMADDYSDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMDTLKQHGAGAGGTRNISGTSKFHVDLERELADLHGKDAALLFSSCFVANDSTLFTLAKMMPGC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aminolevulinate, delta-, synthase 1 |
| 别名 | 5-aminolevulinate synthase, non-specific, mitochondrial, 5-aminolevulinic acid synthase 1, ALAS, Alas 1, ALAS 3 |
| 计算分子量 | 640 aa, 71 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC011798 |
| 基因名称 | ALAS1 |
| Gene ID (NCBI) | 211 |
| RRID | AB_2878227 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13196 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ALAS1 antibody 16200-1-AP | Download protocol |
| WB protocol for ALAS1 antibody 16200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. | ||
Naunyn Schmiedebergs Arch Pharmacol Role of aminolevulinic acid synthase 1 in doxorubicin-induced oxidative stress to the ardiomyocyte. | ||
Saudi J Gastroenterol Inhibition of ALAS1 activity exerts anti-tumour effects on colorectal cancer in vitro. | ||
Evid Based Complement Alternat Med Genetic and Functional Evaluation of the Role of FOXO1 in Antituberculosis Drug-Induced Hepatotoxicity. | ||
Arq Bras Cardiol Effects of Doxorubicin on Heme Biosynthesis and Metabolism in Cardiomyocyte. |
