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ALAS1 Polyclonal antibody

ALAS1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16200-1-AP

Print datasheet

Synonyms

ALAS, ALAS H, ALAS1, ALAS3, ALASH, Delta ALA synthase 1, MIG4, Mitochondrion Marker

验证数据展示

  • WB analysis using 16200-1-AP

    WB analysis using 16200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16200-1-AP (ALAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16200-1-AP

    IF Staining of HepG2 using 16200-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16200-1-AP (ALAS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Raji cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16200-1-AP targets ALAS1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ALAS1 fusion protein Ag9126 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aminolevulinate, delta-, synthase 1
Synonyms ALAS, ALAS H, ALAS1, ALAS3, ALASH, Delta ALA synthase 1, MIG4, Mitochondrion Marker
Calculated Molecular Weight 640 aa, 71 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC011798
Gene Symbol ALAS1
Gene ID (NCBI) 211
RRIDAB_2878227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13196
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALAS1 antibody 16200-1-APDownload protocol
IF protocol for ALAS1 antibody 16200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Authors - Ying Wang
View Article
humanWB

Mol Cell

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Authors - Gokhan Unlu
View Article
ratWB

Naunyn Schmiedebergs Arch Pharmacol

Role of aminolevulinic acid synthase 1 in doxorubicin-induced oxidative stress to the ardiomyocyte.

Authors - Zuoyan Wang
View Article
humanIF

Saudi J Gastroenterol

Inhibition of ALAS1 activity exerts anti-tumour effects on colorectal cancer in vitro.

Authors - Yalei Zhao
View Article
humanWB

Evid Based Complement Alternat Med

Genetic and Functional Evaluation of the Role of FOXO1 in Antituberculosis Drug-Induced Hepatotoxicity.

Authors - Jingwei Zhang
View Article
humanWB

Arq Bras Cardiol

Effects of Doxorubicin on Heme Biosynthesis and Metabolism in Cardiomyocyte.

Authors - Zuoyan Wang
View Article