验证数据展示
经过测试的应用
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-28113 targets AKT2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag27005 Product name: Recombinant human AKT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 335-481 aa of BC120994 Sequence: GLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLSPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIRE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-akt murine thymoma viral oncogene homolog 2 |
| 别名 | AKT 2, AKT2 protein, EC:2.7.11.1, PKB beta, PKBB |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC120994 |
| 基因名称 | AKT2 |
| Gene ID (NCBI) | 208 |
| RRID | AB_3672822 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31751 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 AKT2 antibody CL488-28113 | Download protocol |
| IF protocol for CL Plus 488 AKT2 antibody CL488-28113 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
