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验证数据展示

  • WB analysis of Jurkat using 14622-1-AP

    WB analysis of Jurkat using 14622-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14622-1-AP (AKAP14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14622-1-AP

    IHC staining of human kidney using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 14622-1-AP

    IHC staining of human kidney using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

AKAP14 Polyclonal antibody

AKAP14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14622-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

A kinase anchor protein 14, A kinase anchor protein 28 kDa, AKAP 14, AKAP 28, AKAP14, AKAP28

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, human testis tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14622-1-AP targets AKAP14 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6255

Product name: Recombinant human AKAP14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-197 aa of BC066357

Sequence: MSETQNSTSQKAMDEDNKAASQTMPNTQDKNYEDELTQVALALVEDVINYAVKIVEEERNPLKNIKWMTHGEFTVEKGLKQIDEYFSKCVSKKCWAHGVEFVERKDLIHSFLYIYYVHWSISTADLPVARISAGTYFTMKVSKTKPPDAPIVVSYVGDHQALVHRPGMVRFRENWQKNLTDAKYSFMESFPFLFNRV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 14
别名 A kinase anchor protein 14, A kinase anchor protein 28 kDa, AKAP 14, AKAP 28, AKAP14, AKAP28
计算分子量 23 kDa
观测分子量 28 kDa
GenBank蛋白编号BC066357
基因名称 AKAP14
Gene ID (NCBI) 158798
RRIDAB_2225760
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UN6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for AKAP14 antibody 14622-1-APDownload protocol
WB protocol for AKAP14 antibody 14622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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