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验证数据展示

  • WB analysis using 85119-2-RR (same clone as 85119-2-PBS)

    WB analysis using 85119-2-RR (same clone as 85119-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85119-2-RR (AKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85119-2-RR (same clone as 85119-2-PBS)

    IF Staining of HepG2 using 85119-2-RR (same clone as 85119-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

    IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85119-2-RR (AKAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

    IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85119-2-RR (AKAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 85119-2-RR (same clone as 85119-2-PBS)

    FC experiment of HEK-293T using 85119-2-RR (same clone as 85119-2-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.13 ug AKAP1 Recombinant antibody (85119-2-RR, Clone:242763A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85119-2-RR

    Affinity and Kinetic Characterization of 85119-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85119-2-RR against Human AKAP1 were performed. The affinity constant is 3.62 nM.

AKAP1 Recombinant monoclonal antibody, PBS Only

AKAP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85119-2-PBS

产品说明书

CloneNo. 242763A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

242763A5, AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85119-2-PBS targets AKAP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8037

Product name: Recombinant human AKAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 31-351 aa of BC000729

Sequence: GHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 1
别名 242763A5, AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa
计算分子量 97 kDa
观测分子量149 kDa
GenBank蛋白编号BC000729
基因名称 AKAP1
Gene ID (NCBI) 8165
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92667
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

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