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验证数据展示

  • WB analysis of human spleen using 66262-1-Ig (same clone as 66262-1-PBS)

    WB analysis of human spleen using 66262-1-Ig (same clone as 66262-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66262-1-Ig (same clone as 66262-1-PBS)

    IF Staining of HeLa using 66262-1-Ig (same clone as 66262-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIRE antibody (66262-1-Ig, Clone: 1A3C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66262-1-PBS in a different storage buffer formulation.

AIRE Monoclonal antibody, PBS Only

AIRE Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66262-1-PBS

产品说明书

CloneNo. 1A3C3

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IF/ICC, Indirect ELISA

别名

PGA1, APSI, APS1, APECED, AIRE1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AIRE Ready-To-Use IHC Kit. AIRE primary antibody included.

产品信息

66262-1-PBS targets AIRE in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag19103

Product name: Recombinant human AIRE protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 96-348 aa of BC137270

Sequence: QKNEDECAVCRDGGELICCDGCPRAFHLACLSPPLREIPSGTWRCSSCLQATVQEVQPRAEEPRPQEPPVETPLPPGLRSAGEEVRGPPGEPLAGMDTTLVYKHLPAPPSAAPLPGLDSSALHPLLCVGPEGQQNLAPGARCGVCGDGTDVLRCTHCAAAFHWRCHFPAGTSRPGTGLRCRSCSGDVTPAPVEGVLAPSPARLAPGPAKDDTASHEPALHRDDLESLLSEHTFDGILQWAIQSMARPAAPFPS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 autoimmune regulator
别名 PGA1, APSI, APS1, APECED, AIRE1
计算分子量 545 aa, 58 kDa
观测分子量 58-69 kDa
GenBank蛋白编号BC137270
基因名称 AIRE
Gene ID (NCBI) 326
RRIDAB_2881649
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO43918
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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