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验证数据展示

  • WB analysis of human spleen using 66262-1-Ig

    WB analysis of human spleen using 66262-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66262-1-Ig

    IF Staining of HeLa using 66262-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIRE antibody (66262-1-Ig, Clone: 1A3C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

AIRE Monoclonal antibody

AIRE Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66262-1-Ig

产品说明书

CloneNo. 1A3C3

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IF/ICC, ELISA

别名

PGA1, APSI, APS1, APECED, AIRE1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inhuman spleen tissue, pig spleen tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AIRE Ready-To-Use IHC Kit. AIRE primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66262-1-Ig targets AIRE in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag19103

Product name: Recombinant human AIRE protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 96-348 aa of BC137270

Sequence: QKNEDECAVCRDGGELICCDGCPRAFHLACLSPPLREIPSGTWRCSSCLQATVQEVQPRAEEPRPQEPPVETPLPPGLRSAGEEVRGPPGEPLAGMDTTLVYKHLPAPPSAAPLPGLDSSALHPLLCVGPEGQQNLAPGARCGVCGDGTDVLRCTHCAAAFHWRCHFPAGTSRPGTGLRCRSCSGDVTPAPVEGVLAPSPARLAPGPAKDDTASHEPALHRDDLESLLSEHTFDGILQWAIQSMARPAAPFPS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 autoimmune regulator
别名 PGA1, APSI, APS1, APECED, AIRE1
计算分子量 545 aa, 58 kDa
观测分子量 58-69 kDa
GenBank蛋白编号BC137270
基因名称 AIRE
Gene ID (NCBI) 326
RRIDAB_2881649
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO43918
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for AIRE antibody 66262-1-IgDownload protocol
WB protocol for AIRE antibody 66262-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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