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验证数据展示

  • WB analysis using CL488-66902

    WB analysis using CL488-66902

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66902 (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using CL488-66902

    IF Staining of MCF-7 using CL488-66902

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 AIM2 antibody (CL488-66902, Clone: 3E12A8 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB, IF/ICC
Cat No. CL488-66902

产品说明书

CloneNo. 3E12A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IF/ICC

别名

3E12A8, Interferon-inducible protein AIM2, PYHIN4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy AIM2 Ready-To-Use IHC Kit. AIM2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66902 targets AIM2 in WB, IF/ICC applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IF/ICC Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 AIM2 fusion protein Ag14607 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 absent in melanoma 2
别名 3E12A8, Interferon-inducible protein AIM2, PYHIN4
计算分子量 343 aa, 39 kDa
观测分子量 37-39 and 53 kDa
GenBank蛋白编号BC010940
基因名称 AIM2
Gene ID (NCBI) 9447
RRIDAB_3084284
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14862
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 AIM2 antibody CL488-66902Download protocol
IF protocol for CL Plus 488 AIM2 antibody CL488-66902Download protocol
Standard Protocols
Click here to view our Standard Protocols