AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit and More (1)

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

3E12A8

Cat No : 66902-1-Ig

Print datasheet

Synonyms

absent in melanoma 2, AIM2, PYHIN4



经过测试的应用

Positive WB detected inRAW 264.7 cells, rabbit spleen tissue, human heart tissue, Sp2/0 cells
Positive IHC detected inhuman urothelial carcinoma tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AIM2 Ready-To-Use IHC Kit. AIM2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66902-1-Ig targets AIM2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications IHC, WB, ELISA
Cited Applications CoIP, IF, WB
Tested Reactivity Human, mouse, rat, rabbit
Cited Reactivityhuman, mouse
Immunogen AIM2 fusion protein Ag14607 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name absent in melanoma 2
Synonyms absent in melanoma 2, AIM2, PYHIN4
Calculated Molecular Weight 343 aa, 39 kDa
Observed Molecular Weight 37-39 and 53 kDa
GenBank Accession NumberBC010940
Gene Symbol AIM2
Gene ID (NCBI) 9447
RRIDAB_2882230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14862
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

实验方案

Product Specific Protocols
WB protocol for AIM2 antibody 66902-1-IgDownload protocol
IHC protocol for AIM2 antibody 66902-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

Gut

STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis.

Authors - Ruby E Dawson
humanWB

Theranostics

Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy

Authors - Qingyu Liang
humanWB

Front Oncol

PKIB involved in the metastasis and survival of osteosarcoma

Authors - Rongxue Wan
mouseWB

Cell Commun Signal

Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia.

Authors - Dong-Ming Wu
mouseWB

Sci Total Environ

Cadmium exposure induces pyroptosis in testicular tissue by increasing oxidative stress and activating the AIM2 inflammasome pathway.

Authors - Jinzhao Zhou
mouseWB,IF

EMBO J

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses

Authors - Hao Liu
  • KD Validated