验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, rabbit spleen tissue, human heart tissue, Sp2/0 cells |
| Positive IHC detected in | human urothelial carcinoma tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66902-1-Ig targets AIM2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat, rabbit |
| 文献引用反应性 | human, mouse |
| 免疫原 | AIM2 fusion protein Ag14607 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | absent in melanoma 2 |
| 别名 | PYHIN4, Interferon-inducible protein AIM2, 3E12A8 |
| 计算分子量 | 343 aa, 39 kDa |
| 观测分子量 | 37-39 and 53 kDa |
| GenBank蛋白编号 | BC010940 |
| 基因名称 | AIM2 |
| Gene ID (NCBI) | 9447 |
| RRID | AB_2882230 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O14862 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AIM2 antibody 66902-1-Ig | Download protocol |
| IHC protocol for AIM2 antibody 66902-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gut STAT3-mediated upregulation of the AIM2 DNA sensor links innate immunity with cell migration to promote epithelial tumourigenesis. | ||
Theranostics Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy | ||
Inflamm Res Transcriptomic analysis reveals molecular characterization and immune landscape of PANoptosis-related genes in atherosclerosis | ||
Cell Commun Signal Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia. | ||
Cell Death Discov Tumors cells with mismatch repair deficiency induce hyperactivation of pyroptosis resistant to cell membrane damage but are more sensitive to co-treatment of IFN-γ and TNF-α to PANoptosis |
