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验证数据展示

  • WB analysis using 86156-1-RR (same clone as 86156-1-PBS)

    WB analysis using 86156-1-RR (same clone as 86156-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86156-1-RR (AIF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86156-1-PBS in a different storage buffer formulation.

  • Sample test of MP02287-1

    Sample test of MP02287-1

    The mean AIF concentration was determined to be 20.2 ng/mL in Hela cell extract based on a 0.9 mg/mL extract load.

  • IF Staining of HeLa using 86156-1-RR (same clone as 86156-1-PBS)

    IF Staining of HeLa using 86156-1-RR (same clone as 86156-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIF antibody (86156-1-RR, Clone: 250772G11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86156-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02287-1

    Cytometric bead array standard curve of MP02287-1

    Cytometric bead array standard curve of MP02287-1, AIF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86156-2-PBS. Detection antibody: 86156-1-PBS. Standard: Ag13268. Range: 0.781-100 ng/mL.

AIF Recombinant antibody, PBS Only (Detector)

AIF Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 86156-1-PBS

产品说明书

CloneNo. 250772G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

别名

AIFM1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, PDCD8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AIFM1 Ready-To-Use IHC Kit. AIFM1 primary antibody included.

产品信息

86156-1-PBS targets AIF as part of a matched antibody pair:

MP02287-1: 86156-2-PBS capture and 86156-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 AIF fusion protein Ag13268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apoptosis-inducing factor, mitochondrion-associated, 1
别名 AIFM1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, PDCD8
计算分子量 609 aa, 66 kDa
观测分子量67 kDa
GenBank蛋白编号BC111065
基因名称 AIF
Gene ID (NCBI) 9131
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95831
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).