验证数据展示
产品信息
86156-1-PBS targets AIF as part of a matched antibody pair:
MP02287-1: 86156-2-PBS capture and 86156-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
经测试应用 | WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | AIF fusion protein Ag13268 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | apoptosis-inducing factor, mitochondrion-associated, 1 |
别名 | AIFM1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, PDCD8 |
计算分子量 | 609 aa, 66 kDa |
观测分子量 | 67 kDa |
GenBank蛋白编号 | BC111065 |
基因名称 | AIF |
Gene ID (NCBI) | 9131 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O95831 |
储存缓冲液 | PBS only, pH 7.3. |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).