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验证数据展示

  • WB analysis using 15642-1-AP (same clone as 15642-1-PBS)

    WB analysis using 15642-1-AP (same clone as 15642-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15642-1-AP (AID antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15642-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 15642-1-AP (same clone as 15642-1-PBS)

    IF Staining of human tonsillitis using 15642-1-AP (same clone as 15642-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using AID antibody (15642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15642-1-PBS in a different storage buffer formulation.

AID Polyclonal antibody, PBS Only

AID Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 15642-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, Indirect ELISA

别名

AICDA, Activation-induced cytidine deaminase, ARP2, CDA2, Cytidine aminohydrolase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15642-1-PBS targets AID in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8136

Product name: Recombinant human AID protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-198 aa of BC006296

Sequence: MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 activation-induced cytidine deaminase
别名 AICDA, Activation-induced cytidine deaminase, ARP2, CDA2, Cytidine aminohydrolase
计算分子量 198 aa, 24 kDa
观测分子量24 kDa
GenBank蛋白编号BC006296
基因名称 AIDA/AID
Gene ID (NCBI) 57379
RRIDAB_2224236
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZX7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AID is essential to all three genetic alterations required for antigen-specific immunoglobulin: class switch recombination (CSR), somatic bypermutation (SHM), and gene conversion. Expression of AID is normally restricted to B cells in the GC, where SHM and CSR occur. It is likely these represent AID monomer and homodimer that western blot detected two bands in purified AID from nuclear extracts, one at 52 kDa and the other at 26 kDa. (PMID:16280388)

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