• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 15642-1-AP

    WB analysis using 15642-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15642-1-AP (AID antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of human tonsillitis using 15642-1-AP

    IF Staining of human tonsillitis using 15642-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using AID antibody (15642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AID Polyclonal antibody

AID Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 15642-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

AICDA, Activation-induced cytidine deaminase, ARP2, CDA2, Cytidine aminohydrolase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMG-63 cells, Ramos cells, THP-1 cells, U2OS cells, NIH/3T3 cells
Positive IF-P detected inhuman tonsillitis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15642-1-AP targets AID in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8136

Product name: Recombinant human AID protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-198 aa of BC006296

Sequence: MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 activation-induced cytidine deaminase
别名 AICDA, Activation-induced cytidine deaminase, ARP2, CDA2, Cytidine aminohydrolase
计算分子量 198 aa, 24 kDa
观测分子量24 kDa
GenBank蛋白编号BC006296
基因名称 AIDA/AID
Gene ID (NCBI) 57379
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZX7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AID is essential to all three genetic alterations required for antigen-specific immunoglobulin: class switch recombination (CSR), somatic bypermutation (SHM), and gene conversion. Expression of AID is normally restricted to B cells in the GC, where SHM and CSR occur. It is likely these represent AID monomer and homodimer that western blot detected two bands in purified AID from nuclear extracts, one at 52 kDa and the other at 26 kDa. (PMID:16280388)

实验方案

Product Specific Protocols
IF protocol for AID antibody 15642-1-APDownload protocol
WB protocol for AID antibody 15642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...