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验证数据展示

  • WB analysis using 13643-1-AP (same clone as 13643-1-PBS)

    WB analysis using 13643-1-AP (same clone as 13643-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13643-1-AP (AHSP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13643-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 13643-1-AP (same clone as 13643-1-PBS)

    IHC staining of human placenta using 13643-1-AP (same clone as 13643-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13643-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 13643-1-AP (same clone as 13643-1-PBS)

    IHC staining of mouse lung using 13643-1-AP (same clone as 13643-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13643-1-PBS in a different storage buffer formulation.

AHSP Polyclonal antibody, PBS Only

AHSP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13643-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig

应用

WB, IHC, Indirect ELISA

别名

ERAF, Erythroid-associated factor, Erythroid differentiation-related factor, erythroid associated factor, EDRF

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13643-1-PBS targets AHSP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, pig
免疫原

CatNo: Ag4588

Product name: Recombinant human ERAF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-102 aa of BC035842

Sequence: MALLKANKDLISAGLKEFSVLLNQQVFNDPLVSEEDMVTVVEDWMNFYINYYRQQVTGEPQERDKALQELRQELNTLANPFLAKYRDFLKSHELPSHPPPSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 erythroid associated factor
别名 ERAF, Erythroid-associated factor, Erythroid differentiation-related factor, erythroid associated factor, EDRF
计算分子量 102 aa, 12 kDa
观测分子量 10 kDa
GenBank蛋白编号BC035842
基因名称 AHSP
Gene ID (NCBI) 51327
RRIDAB_3669167
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZD4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development, and specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia (PMID: 12066189).

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