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验证数据展示

  • WB analysis using 13643-1-AP

    WB analysis using 13643-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13643-1-AP (AHSP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13643-1-AP

    IHC staining of human placenta using 13643-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 13643-1-AP

    IHC staining of mouse lung using 13643-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AHSP Polyclonal antibody

AHSP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13643-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig

应用

WB, IHC, ELISA

别名

ERAF, Erythroid-associated factor, Erythroid differentiation-related factor, erythroid associated factor, EDRF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig bone marrow tissue
Positive IHC detected inhuman placenta tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13643-1-AP targets AHSP in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, pig
免疫原 AHSP fusion protein Ag4588 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 erythroid associated factor
别名 ERAF, Erythroid-associated factor, Erythroid differentiation-related factor, erythroid associated factor, EDRF
计算分子量 102 aa, 12 kDa
观测分子量 10 kDa
GenBank蛋白编号BC035842
基因名称 AHSP
Gene ID (NCBI) 51327
RRIDAB_3669167
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZD4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development, and specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia (PMID: 12066189).

实验方案

Product Specific Protocols
WB protocol for AHSP antibody 13643-1-APDownload protocol
IHC protocol for AHSP antibody 13643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols