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验证数据展示

  • WB analysis using 68592-1-Ig

    WB analysis using 68592-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68592-1-Ig (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 68592-1-Ig

    IF Staining of A431 using 68592-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using AEG-1/MTDH antibody (68592-1-Ig, Clone: 1D8C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IHC staining of human rectal cancer using 68592-1-Ig

    IHC staining of human rectal cancer using 68592-1-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTDH antibody included
  • IHC staining of human rectal cancer using 68592-1-Ig

    IHC staining of human rectal cancer using 68592-1-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTDH antibody included
  • IHC staining of human tonsillitis using 68592-1-Ig

    IHC staining of human tonsillitis using 68592-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTDH antibody included
  • IHC staining of human rectal cancer using 68592-1-Ig

    IHC staining of human rectal cancer using 68592-1-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTDH antibody included

AEG-1/MTDH Monoclonal antibody

AEG-1/MTDH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68592-1-Ig

产品说明书

CloneNo. 1D8C2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

AEG-1, MTDH, 1D8C2, 3D3/LYRIC, AEG 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, MCF-10A cells, A549 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inhuman rectal cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MTDH Ready-To-Use IHC Kit. MTDH primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68592-1-Ig targets AEG-1/MTDH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5033

Product name: Recombinant human AEG-1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 233-582 aa of BC045642

Sequence: EQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKAEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 metadherin
别名 AEG-1, MTDH, 1D8C2, 3D3/LYRIC, AEG 1
计算分子量 582 aa, 64 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC045642
基因名称 AEG-1/MTDH
Gene ID (NCBI) 92140
RRIDAB_3085291
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ86UE4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.

实验方案

Product Specific Protocols
IF protocol for AEG-1/MTDH antibody 68592-1-IgDownload protocol
IHC protocol for AEG-1/MTDH antibody 68592-1-IgDownload protocol
WB protocol for AEG-1/MTDH antibody 68592-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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