验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13096-1-AP targets ADRB2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3715 Product name: Recombinant human ADRB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 220-413 aa of BC012481 Sequence: SRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSPGRNCSTNDSLL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adrenergic, beta-2-, receptor, surface |
| 别名 | ADRB2, ADRB2R, ADRBR, B2AR, BAR, Beta 2 adrenergic receptor, Beta 2 adrenoceptor, Beta 2 adrenoreceptor, BETA2AR |
| 计算分子量 | 46 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC012481 |
| 基因名称 | ADRB2 |
| Gene ID (NCBI) | 154 |
| RRID | AB_2225401 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07550 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-2 adrenergic receptor (ADRB2), a member of the G protein-coupled receptor (GPCR) family, binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. ADRB2 is directly linked to one of its final effectors, the class C L-type Ca2+ channel, Cav1.2 (PMID: 11441182). This receptor-channel complex also contains a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase (PKA), and a counteracting phosphatase, PP2A. Different polymorphic forms, point mutations, and/or downregulation of the gene of ADRB2 are associated with nocturnal asthma, obesity and type 2 diabetes. This antibody detects ADRB2 with an apparent molecular weight of 50-55 kDa as has been shown by some researches (PMID: 25348166; 12502786; 29232706; 23984976; 19584355).
发表文章
| Species | Application | Title |
|---|---|---|
Bone Res Inhibition of sympathetic tone via hypothalamic descending pathway propagates glucocorticoid-induced endothelial impairment and osteonecrosis of the femoral head | ||
Cell Commun Signal Olanzapine suppresses mPFC activity-norepinephrine releasing to alleviate CLOCK-enhanced cancer stemness under chronic stress
| ||
J Transl Med Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis. | ||
Proc Natl Acad Sci U S A Astrocytic β2-adrenergic receptors mediate hippocampal long-term memory consolidation. | ||
Diabetes Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination. | ||
Mol Ther Nucleic Acids Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes. |
