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ADPGK Polyclonal antibody

ADPGK Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15639-1-AP

Print datasheet

Synonyms

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, RbBP 35

验证数据展示

  • WB analysis using 15639-1-AP

    WB analysis using 15639-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15639-1-AP (ADPGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15639-1-AP

    IHC staining of human liver using 15639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 15639-1-AP

    IHC staining of human liver using 15639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 15639-1-AP

    IF Staining of HepG2 using 15639-1-AP

    Immunofluorescent analysis of HepG2 cells using 15639-1-AP (ADPGK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15639-1-AP targets ADPGK in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ADPGK fusion protein Ag8105 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-dependent glucokinase
Synonyms 2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, RbBP 35
Calculated Molecular Weight 497 aa, 54 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC006112
Gene Symbol ADPGK
Gene ID (NCBI) 83440
RRIDAB_2224911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

实验方案

Product Specific Protocols
WB protocol for ADPGK antibody 15639-1-APDownload protocol
IHC protocol for ADPGK antibody 15639-1-APDownload protocol
IF protocol for ADPGK antibody 15639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Front Mol Neurosci

Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis.

Authors - Kai Zhang
View Article