验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, human brain tissue, mouse spleen tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
产品信息
16479-1-AP targets ADO in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag9598 Product name: Recombinant human ADO protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-270 aa of BC018660 Sequence: MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGGGQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAPPYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 2-aminoethanethiol (cysteamine) dioxygenase |
| 别名 | 2 aminoethanethiol dioxygenase, 2-aminoethanethiol dioxygenase, C10orf22, Cysteamine dioxygenase, EC:1.13.11.19 |
| 计算分子量 | 270 aa, 30 kDa |
| 观测分子量 | 28 kDa-30 kDa |
| GenBank蛋白编号 | BC018660 |
| 基因名称 | ADO |
| Gene ID (NCBI) | 84890 |
| RRID | AB_2222204 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96SZ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADO, or 2-aminoethanethiol dioxygenase, cysteamine dioxygenase, is a thiol dioxygenase in humans that adds two oxygen atoms to cysteamine, forming hypotaurine (PMID: 39637922). ADO has been reported to exhibit two distinct biological functions with a nonheme iron center. It catalyzes oxidation of both cysteamine in sulfur metabolism and N-terminal cysteine-containing proteins or peptides, such as regulator of G protein signaling 5 (RGS5) (PMID: 34508780). ADO is ubiquitously expressed with the highest levels in brain, heart, and skeletal muscle. Western blot analysis detected two bands at 28~30 kDa as indicated in publications (PMID: 17581819).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADO antibody 16479-1-AP | Download protocol |
| WB protocol for ADO antibody 16479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomol Ther (Seoul) Taurine Synthesis by 2-Aminoethanethiol Dioxygenase as a Vulnerable Metabolic Alteration in Pancreatic Cancer
| ||
Cell Death Discov ADO/hypotaurine: a novel metabolic pathway contributing to glioblastoma development.
| ||
Free Radic Biol Med Taurine ameliorates radiation-induced oxidative stress in bone marrow mesenchymal stromal cells and promotes osteogenesis |
