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验证数据展示

  • WB analysis using 13288-2-AP

    WB analysis using 13288-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13288-2-AP (ADH6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 13288-2-AP

    IHC staining of human liver using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADH6 antibody included
  • IHC staining of mouse kidney using 13288-2-AP

    IHC staining of mouse kidney using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADH6 antibody included
  • IHC staining of rat kidney using 13288-2-AP

    IHC staining of rat kidney using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADH6 antibody included
  • IF Staining of MCF-7 using 13288-2-AP

    IF Staining of MCF-7 using 13288-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ADH6 antibody (13288-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ADH6 Polyclonal antibody

ADH6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13288-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:1.1.1.1, ADH-5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, human liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ADH6 Ready-To-Use IHC Kit. ADH6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13288-2-AP targets ADH6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ADH6 fusion protein Ag3984 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alcohol dehydrogenase 6 (class V)
别名 EC:1.1.1.1, ADH-5
计算分子量 368 aa, 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC039065
基因名称 ADH6
Gene ID (NCBI) 130
RRIDAB_2273710
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28332
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADH6 antibody 13288-2-APDownload protocol
IHC protocol for ADH6 antibody 13288-2-APDownload protocol
IF protocol for ADH6 antibody 13288-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols