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验证数据展示

  • WB analysis of HeLa using 14330-1-AP (same clone as 14330-1-PBS)

    WB analysis of HeLa using 14330-1-AP (same clone as 14330-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 14330-1-AP (same clone as 14330-1-PBS)

    IHC staining of human colon cancer using 14330-1-AP (same clone as 14330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADAR1 antibody included
  • IHC staining of human colon cancer using 14330-1-AP (same clone as 14330-1-PBS)

    IHC staining of human colon cancer using 14330-1-AP (same clone as 14330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADAR1 antibody included
  • WB analysis using 14330-1-AP (same clone as 14330-1-PBS)

    WB analysis using 14330-1-AP (same clone as 14330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 14330-1-AP (same clone as 14330-1-PBS)

    IP experiment of HepG2 using 14330-1-AP (same clone as 14330-1-PBS)

    IP result of anti-ADAR1 (IP:14330-1-AP, 4ug; Detection:14330-1-AP 1:800) with HepG2 cells lysate 1320 ug. This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14330-1-AP (same clone as 14330-1-PBS)

    IF Staining of HeLa using 14330-1-AP (same clone as 14330-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADAR1 antibody (14330-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ADAR1 Polyclonal antibody, PBS Only

ADAR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14330-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

ADAR, 136 kDa double-stranded RNA-binding protein, adenosine deaminase RNA specific, Double-stranded RNA-specific adenosine deaminase, DRADA

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ADAR1 Ready-To-Use IHC Kit. ADAR1 primary antibody included.

产品信息

14330-1-PBS targets ADAR1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag5609

Product name: Recombinant human ADAR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 877-1226 aa of BC038227

Sequence: DSEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFKKGLKDMGYGNWISKPQEEKNFYLCPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenosine deaminase, RNA-specific
别名 ADAR, 136 kDa double-stranded RNA-binding protein, adenosine deaminase RNA specific, Double-stranded RNA-specific adenosine deaminase, DRADA
计算分子量 136 kDa
观测分子量 110 kDa
GenBank蛋白编号BC038227
基因名称 ADAR1
Gene ID (NCBI) 103
RRIDAB_2273600
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55265
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).

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