验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human colon cancer tissue, human gliomas tissue, human stomach cancer tissue, mouse brain tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14330-1-AP targets ADAR1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5609 Product name: Recombinant human ADAR1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 877-1226 aa of BC038227 Sequence: DSEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFKKGLKDMGYGNWISKPQEEKNFYLCPV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adenosine deaminase, RNA-specific |
| 别名 | ADAR, 136 kDa double-stranded RNA-binding protein, adenosine deaminase RNA specific, Double-stranded RNA-specific adenosine deaminase, DRADA |
| 计算分子量 | 136 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC038227 |
| 基因名称 | ADAR1 |
| Gene ID (NCBI) | 103 |
| RRID | AB_2273600 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55265 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ADAR1 antibody 14330-1-AP | Download protocol |
| IHC protocol for ADAR1 antibody 14330-1-AP | Download protocol |
| IP protocol for ADAR1 antibody 14330-1-AP | Download protocol |
| WB protocol for ADAR1 antibody 14330-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator
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Nat Commun All-trans retinoic acid destabilizes ADAR1 protein through retinoylation-mediated USP7 dissociation and improves immunotherapy in pancreatic cancer. | ||
Redox Biol ADAR1 exacerbates ischemic brain injury via astrocyte-mediated neuron apoptosis | ||
Nucleic Acids Res Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression | ||
Genes Dis 8-Chloroadenosine suppresses hepatocellular carcinoma progression via ADAR1/PPARγ axis-mediated lipid metabolism.
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