验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue, rat cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32969-1-AP targets ADAM23 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag35744 Product name: Recombinant human ADAM23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 342-488 aa of BC132765 Sequence: NTRVVLVAVETWTEKDQIDITTNPVQMLHEFSKYRQRIKQHADAVHLISRVTFHYKRSSLSYFGGVCSRTRGVGVNEYGLPMAVAQVLSQSLAQNLGIQWEPSSRKPKCDCTESWGGCIMEETGVSHSRKFSKCSILEYRDFLQRGG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADAM metallopeptidase domain 23 |
| 别名 | ADAM 23, Disintegrin and metalloproteinase domain-containing protein 23, MDC3, MDC-3, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3 |
| 计算分子量 | 832 aa, 92 kDa |
| 观测分子量 | 92 kDa, 70 kDa |
| GenBank蛋白编号 | BC132765 |
| 基因名称 | ADAM23 |
| Gene ID (NCBI) | 8745 |
| RRID | AB_3742807 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O75077 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADAM23 (A disintegrin and metalloprotease protein 23), also known as MDC-3 (Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3) is a transmembrane type I glycoprotein involved with the development and maintenance of the nervous system, including neurite outgrowth, neuronal adhesion and differentiation and regulation of synaptic transmission. In addition, ADAM23 seems to participate in immune response and tumor establishment through interaction with different members of integrin receptors (PMID: 19692335). Two forms of ADAM23 have been previously described in primary cerebellar granule cells as an unprocessed precursor (100 kDa) and mature (70 kDa) protein, respectively (PMID: 29792904).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAM23 antibody 32969-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
