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验证数据展示

  • FC experiment of human PBMCs using 98674-2-RR

    FC experiment of human PBMCs using 98674-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ADAM17 Rabbit RecAb (98674-2-RR, Clone:251631A5), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98674-2-RR

    FC experiment of human PBMCs using 98674-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ADAM17 Rabbit RecAb (98674-2-RR, Clone:251631A5) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity of 98674-2-RR

    Affinity of 98674-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98674-2-RR against Human ADAM17 were performed. The affinity constant is 1.60 nM.

Anti-Human ADAM17 Rabbit Recombinant Antibody

ADAM17 Uni-rAb® Recombinant Antibody for FC
Cat No. 98674-2-RR

产品说明书

CloneNo. 251631A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86, Snake venom-like protease

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ADAM17 Ready-To-Use IHC Kit. ADAM17 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98674-2-RR targets ADAM17 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg4732

Product name: Recombinant Human ADAM17 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 470-570 aa of NM_003183.5

Sequence: FQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKEGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGNAEDDTVCLDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ADAM metallopeptidase domain 17
别名 ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86, Snake venom-like protease
计算分子量93 kDa
GenBank蛋白编号NM_003183.5
基因名称 ADAM17
Gene ID (NCBI) 6868
RRIDAB_3746262
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78536-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 mediates the release of pro-inflammatory cytokines and modulates cell signaling pathways involved in tumor progression and metastasis (PMID:17438092).

实验方案

Product Specific Protocols
FC protocol for ADAM17 antibody 98674-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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