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验证数据展示

  • IF Staining of HepG2 using CL488-68037

    IF Staining of HepG2 using CL488-68037

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ACTR3B antibody (CL488-68037, Clone: 1G12C6 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-68037

    FC experiment of Jurkat using CL488-68037

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ACTR3B (CL488-68037, Clone:1G12C6) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated ACTR3B Monoclonal antibody

ACTR3B Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68037

产品说明书

CloneNo. 1G12C6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 68037-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68037 targets ACTR3B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag10218

Product name: Recombinant human ACTR3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 156-418 aa of BC008682

Sequence: RQVGERTLTGIVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLREREVGIPPEQSLETAKAIKEKYCYICPDIVKEFAKYDVDPRKWIKQYTGINAINQKKFVIDVGYERFLGPEIFFHPEFANPDFMESISDVVDEVIQNCPIDVRRPLYKNVVLSGGSTMFRDFGRRLQRDLKRVVDARLRLSEELSGGRIKPKPVEVQVVTHHMQRYAVWFGGSMLASTPEFFQVCHTKKDYEEYGPSICRHNPVFGVMS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ARP3 actin-related protein 3 homolog B (yeast)
别名 1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B
计算分子量 418 aa, 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC008682
基因名称 ACTR3B
Gene ID (NCBI) 57180
RRIDAB_2923866
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9P1U1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACTR3B antibody CL488-68037Download protocol
Standard Protocols
Click here to view our Standard Protocols
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