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CoraLite® Plus 488-conjugated ACTR3B Monoclonal antibody

ACTR3B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G12C6

Cat No : CL488-68037

Print datasheet

Synonyms

Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, ACTR3B, ARP11, ARP3 beta, ARP3BETA, ARP4, DKFZp686O24114

验证数据展示

  • FC experiment of Jurkat using CL488-68037

    FC experiment of Jurkat using CL488-68037

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ACTR3B (CL488-68037, Clone:1G12C6) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 68037-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68037 targets ACTR3B in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen ACTR3B fusion protein Ag10218 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ARP3 actin-related protein 3 homolog B (yeast)
Synonyms Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, ACTR3B, ARP11, ARP3 beta, ARP3BETA, ARP4, DKFZp686O24114
Calculated Molecular Weight 418 aa, 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC008682
Gene Symbol ACTR3B
Gene ID (NCBI) 57180
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P1U1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ACTR3B antibody CL488-68037Download protocol
Standard Protocols
Click here to view our Standard Protocols