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验证数据展示

  • WB analysis using 68037-1-Ig

    WB analysis using 68037-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68037-1-Ig

    WB analysis using 68037-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse brain using 68037-1-Ig

    IHC staining of mouse brain using 68037-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68037-1-Ig

    IHC staining of mouse brain using 68037-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 68037-1-Ig

    IF Staining of HepG2 using 68037-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ACTR3B antibody (68037-1-Ig, Clone: 1G12C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

ACTR3B Monoclonal antibody

ACTR3B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68037-1-Ig

产品说明书

CloneNo. 1G12C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA

别名

1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inMOLT-4 cells, pig brain tissue, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68037-1-Ig targets ACTR3B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 ACTR3B fusion protein Ag10218 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ARP3 actin-related protein 3 homolog B (yeast)
别名 1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B
计算分子量 418 aa, 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC008682
基因名称 ACTR3B
Gene ID (NCBI) 57180
RRIDAB_2918779
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9P1U1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTR3B antibody 68037-1-IgDownload protocol
IHC protocol for ACTR3B antibody 68037-1-IgDownload protocol
IF protocol for ACTR3B antibody 68037-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols