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验证数据展示

  • FC experiment of HeLa using CL594-66628

    FC experiment of HeLa using CL594-66628

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ACTN4 (CL594-66628, Clone:1E12B8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated ACTN4 Monoclonal antibody

ACTN4 Monoclonal Antibody for FC (Intra)
Cat No. CL594-66628

产品说明书

CloneNo. 1E12B8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

FC (Intra)

别名

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, DKFZp686K23158, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66628-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66628 targets ACTN4 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat
免疫原 ACTN4 fusion protein Ag13549 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 actinin, alpha 4
别名 ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, DKFZp686K23158, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4
计算分子量 105 kDa
观测分子量 105 and 80 kDa
GenBank蛋白编号BC005033
基因名称 ACTN4
Gene ID (NCBI) 81
RRIDAB_3084717
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43707
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

实验方案

Product Specific Protocols
FC protocol for CL594 ACTN4 antibody CL594-66628Download protocol
Standard Protocols
Click here to view our Standard Protocols