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验证数据展示

  • WB analysis of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    WB analysis of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • FC experiment of C2C12 using 17521-1-AP (same clone as 17521-1-PBS)

    FC experiment of C2C12 using 17521-1-AP (same clone as 17521-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTA1-Specific (17521-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

ACTA1-Specific Polyclonal antibody, PBS Only

ACTA1-Specific Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 17521-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

17521-1-PBS targets ACTA1-Specific in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actin, alpha 1, skeletal muscle
别名
计算分子量 42 kDa
观测分子量 39-42 kDa
GenBank蛋白编号NM_001100
基因名称 ACTA1
Gene ID (NCBI) 58
RRIDAB_2273608
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP68133
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.