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验证数据展示

  • WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 16087-1-AP (same clone as 16087-1-PBS)

    IP experiment of HepG2 using 16087-1-AP (same clone as 16087-1-PBS)

    IP result of anti-ACSS2 (IP:16087-1-AP, 4ug; Detection:16087-1-AP 1:500) with HepG2 cells lysate 5600 ug. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

    IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

    IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 synthetase antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ACSS2 Polyclonal antibody, PBS Only

ACSS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16087-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16087-1-PBS targets ACSS2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9121

Product name: Recombinant human ACSS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 185-534 aa of BC010141

Sequence: VFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDVNRLWSIVDKYKVTKFYTAPTAIRLLMKFGDEPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGAQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPKLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDMSTVADPSVISHLFSHRCLTIQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA synthetase short-chain family member 2
别名 ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1
计算分子量 701 aa, 79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC010141
基因名称 ACSS2
Gene ID (NCBI) 55902
RRIDAB_2878216
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR19
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.

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