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验证数据展示

  • WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

    WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 67334-1-Ig (same clone as 67334-1-PBS)

    WB analysis of rat liver using 67334-1-Ig (same clone as 67334-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67334-1-Ig (same clone as 67334-1-PBS)

    IF Staining of HepG2 using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM5 antibody (67334-1-Ig, Clone: 2D5D6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

ACSM5 Monoclonal antibody, PBS Only

ACSM5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67334-1-PBS

产品说明书

CloneNo. 2D5D6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2D5D6, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67334-1-PBS targets ACSM5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10056

Product name: Recombinant human ACSM5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-208 aa of BC016703

Sequence: MRPWLRHLVLQALRNSRAFCGSHGKPAPLPVPQKIVATWEAISLGRQLVPEYFNFAHDVLDVWSRLEEAGHRPPNPAFWWVNGTGAEIKWSFEELGKQSRKAANVLGGACGLQPGDRMMLVLPRLPEWWLVSVACMRTGTVVIPGVTQLTEKDLKYRLQASRAKSIITSDSLAPRVDAISAECPSLQTKLLVSDSSRPGWLNFRELLR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 acyl-CoA synthetase medium-chain family member 5
别名 2D5D6, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2
计算分子量 208 aa, 23 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC016703
基因名称 ACSM5
Gene ID (NCBI) 54988
RRIDAB_2882592
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ6NUN0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

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