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验证数据展示

  • WB analysis of 4T1 using 67334-1-Ig

    WB analysis of 4T1 using 67334-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 67334-1-Ig

    WB analysis of rat liver using 67334-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67334-1-Ig

    IHC staining of human liver cancer using 67334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67334-1-Ig

    IHC staining of human liver cancer using 67334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67334-1-Ig

    IF Staining of HepG2 using 67334-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM5 antibody (67334-1-Ig, Clone: 2D5D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

ACSM5 Monoclonal antibody

ACSM5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67334-1-Ig

产品说明书

CloneNo. 2D5D6
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

ACSM5, FLJ20581, MACS3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in4T1 cells, MDA-MB-231 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67334-1-Ig targets ACSM5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse, Rat
文献引用反应性human
免疫原 ACSM5 fusion protein Ag10056 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 acyl-CoA synthetase medium-chain family member 5
别名 ACSM5, FLJ20581, MACS3
计算分子量 208 aa, 23 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC016703
基因名称 ACSM5
Gene ID (NCBI) 54988
RRIDAB_2882592
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ6NUN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACSM5 antibody 67334-1-IgDownload protocol
IHC protocol for ACSM5 antibody 67334-1-IgDownload protocol
IF protocol for ACSM5 antibody 67334-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Arthritis Res Ther

Integrative analysis of genome-wide DNA methylation and single-nucleotide polymorphism identified ACSM5 as a suppressor of lumbar ligamentum flavum hypertrophy.

Authors - Yanlin Cao
View Article