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验证数据展示

  • WB analysis using 86817-1-RR

    WB analysis using 86817-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86817-1-RR (ACP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 86817-1-RR

    WB analysis of HepG2 using 86817-1-RR

    WB result of ACP1 antibody (86817-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACP1 transfected HepG2 cells.

  • IF Staining of 4T1 using 86817-1-RR

    IF Staining of 4T1 using 86817-1-RR

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using ACP1 antibody (86817-1-RR, Clone: 251838C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86817-1-RR

    Affinity and Kinetic Characterization of 86817-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86817-1-RR against Human ACP1 were performed. The affinity constant is below 1 pM.

  • KD/KO Validated

ACP1 Recombinant monoclonal antibody

ACP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86817-1-RR

产品说明书

CloneNo. 251838C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ACP 1, EC:3.1.3.2, EC:3.1.3.48, HAAP, LMW PTP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, K-562 cells, RAW 264.7 cells, NIH/3T3 cells, PC-12 cells
Positive IF/ICC detected in4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86817-1-RR targets ACP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17539

Product name: Recombinant human ACP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 15-102 aa of BC007422

Sequence: GNICRSPIAEAVFRKLVTDQNISENWVIDSGAVSDWNVGRSPDPRAVSCLRNHGIHTAHKARQITKEDFATFDYILCMDESNLRDLNR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acid phosphatase 1, soluble
别名 ACP 1, EC:3.1.3.2, EC:3.1.3.48, HAAP, LMW PTP
计算分子量 158 aa, 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC007422
基因名称 ACP1
Gene ID (NCBI) 52
RRIDAB_3745140
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP24666
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acid phosphatase 1 (ACP1) is a low-molecular-weight phosphotyrosine protein phosphatase known to participate in various cellular processes, including signal transduction, proliferation, and metabolism. It functions by dephosphorylating tyrosine-phosphorylated proteins, thereby modulating receptor-mediated signaling pathways.

实验方案

Product Specific Protocols
IF protocol for ACP1 antibody 86817-1-RRDownload protocol
WB protocol for ACP1 antibody 86817-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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