验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue, mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85136-1-RR targets ACOX2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11778 Product name: Recombinant human ACOX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-681 aa of BC047700 Sequence: KVLDYQTQQQKLFPQLAISYAFHFLAVSLLEFFQHSYTAILNQDFSFLPELHALSTGMKAMMSEFCTQGAEMCRRACGGHGYSKLSGLPSLVTKLSASCTYEGENTVLYLQVARFLVKSYLQTQMSPGSTPQRSLSPSVAYLTAPDLARCPAQRAADFLCPELYTTAWAHVAVRLIKDSVQHLQTLTQSGADQHEAWNQTTVIHLQAAKVHCYYVTVKGFTEALEKLENEPAIQQVLKRLCDLHAIHGILTNSGDFLHDAFLSGAQVDMARTAYLDLLRLIRKDAILLTDAFDFTDQCLNSALGCYDGNVYERLFQWAQKSPTNTQENPAYEEYIRPLLQSWRSKL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | acyl-Coenzyme A oxidase 2, branched chain |
| 别名 | 242752C7, 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA 24-hydroxylase, 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase, EC:1.17.99.3, Peroxisomal acyl-coenzyme A oxidase 2 |
| 计算分子量 | 681 aa, 77 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC047700 |
| 基因名称 | ACOX2 |
| Gene ID (NCBI) | 8309 |
| RRID | AB_3744042 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99424 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACOX2 (Acyl-CoA Oxidase 2) is a peroxisomal enzyme that catalyzes the first and rate-limiting step of branched-chain fatty acid β-oxidation, specifically targeting very long-chain fatty acids (VLCFAs) and bile acid intermediates. It plays a critical role in lipid metabolism by generating H2O2 and acetyl-CoA, thereby linking peroxisomal fatty acid oxidation to cellular energy homeostasis and redox signaling. Dysregulation of ACOX2 has been implicated in metabolic disorders, including non-alcoholic fatty liver disease (NAFLD) and certain peroxisomal biogenesis disorders, highlighting its potential as a therapeutic target for metabolic and liver-related diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACOX2 antibody 85136-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
