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验证数据展示

  • FC experiment of HepG2 using CL488-68005

    FC experiment of HepG2 using CL488-68005

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ACAT2 (CL488-68005, Clone:1F5F11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-68005

    IF Staining of HepG2 using CL488-68005

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ACAT2 antibody (CL488-68005, Clone: 1F5F11 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ACAT2 Monoclonal antibody

ACAT2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68005

产品说明书

CloneNo. 1F5F11

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit

应用

IF/ICC, FC (Intra)

别名

ACAT2, acetoacetyl CoA thiolase, Acetyl-CoA acetyltransferase, cytosolic, Acetyl-CoA transferase-like protein, ACTL, Cytosolic acetoacetyl-CoA thiolase, EC:2.3.1.9, 1F5F11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68005-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68005 targets ACAT2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat, rabbit samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human, mouse, rat, rabbit
免疫原 ACAT2 fusion protein Ag6596 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 acetyl-Coenzyme A acetyltransferase 2
别名 ACAT2, acetoacetyl CoA thiolase, Acetyl-CoA acetyltransferase, cytosolic, Acetyl-CoA transferase-like protein, ACTL, Cytosolic acetoacetyl-CoA thiolase, EC:2.3.1.9, 1F5F11
计算分子量 41 kDa
观测分子量42 kDa
GenBank蛋白编号BC000408
基因名称 ACAT2
Gene ID (NCBI) 39
RRIDAB_2923855
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BWD1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACAT2 antibody CL488-68005Download protocol
FC protocol for CL Plus 488 ACAT2 antibody CL488-68005Download protocol
Standard Protocols
Click here to view our Standard Protocols