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验证数据展示

  • WB analysis using 60485-4-Ig (same clone as 60485-4-PBS)

    WB analysis using 60485-4-Ig (same clone as 60485-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60485-4-Ig (ACAT1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60485-4-PBS in a different storage buffer formulation.

  • WB analysis using 60485-4-Ig (same clone as 60485-4-PBS)

    WB analysis using 60485-4-Ig (same clone as 60485-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60485-4-Ig (ACAT1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60485-4-PBS in a different storage buffer formulation.

ACAT1 Monoclonal antibody, PBS Only

ACAT1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 60485-4-PBS

产品说明书

CloneNo. 1F3H1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ACAT1 Ready-To-Use IHC Kit. ACAT1 primary antibody included.

产品信息

60485-4-PBS targets ACAT1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9219

Product name: Recombinant human ACAT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-162 aa of BC010942

Sequence: MAVLPALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQIKQETGSLAKICCHVRR

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 acetyl-Coenzyme A acetyltransferase 1
别名 ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9
计算分子量 45 kDa
观测分子量 38-45 kDa
GenBank蛋白编号BC010942
基因名称 ACAT1
Gene ID (NCBI) 38
RRIDAB_3743217
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP24752
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

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