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验证数据展示

  • WB analysis using 29350-1-AP (same clone as 29350-1-PBS)

    WB analysis using 29350-1-AP (same clone as 29350-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29350-1-AP (ABR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29350-1-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 29350-1-AP (same clone as 29350-1-PBS)

    WB analysis of U-251 using 29350-1-AP (same clone as 29350-1-PBS)

    WB result of ABR antibody (29350-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABR transfected U-251 cells. This data was developed using the same antibody clone with 29350-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 29350-1-AP (same clone as 29350-1-PBS)

    IHC staining of rat brain using 29350-1-AP (same clone as 29350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29350-1-AP (ABR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29350-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ABR Polyclonal antibody, PBS Only

ABR Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29350-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

active BCR related gene, MDB

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29350-1-PBS targets ABR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29522

Product name: Recombinant human ABR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-87 aa of NM_021962

Sequence: MEPLSHRGLPRLSWIDTLYSNFSYGTDEYDGEGNEEQKGPPEGSETMPYIDESPTMSPQLSARSQGGGDGVSPTPPEGLAPGVEAGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 active BCR-related gene
别名 active BCR related gene, MDB
计算分子量 98 kDa
观测分子量97-100 kDa
GenBank蛋白编号NM_021962
基因名称 ABR
Gene ID (NCBI) 29
RRIDAB_3086122
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12979
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABR (Active breakpoint cluster region-related protein) is the only protein known in humans and mice to share high homology with BCR (68% amino acid identity). BCR(Breakpoint cluster region) gene was originally identified due to its involvement in a specific chromosomal translocation that causes the development of chronic myeloid leukemia and a subset of acute lymphoblastic leukemia. The C-terminus is a GTPase-activating protein domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. (PMID: 17116687, PMID: 37507586)

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