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验证数据展示

  • WB analysis using 29350-1-AP

    WB analysis using 29350-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29350-1-AP (ABR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-251 using 29350-1-AP

    WB analysis of U-251 using 29350-1-AP

    WB result of ABR antibody (29350-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABR transfected U-251 cells.

  • IHC staining of rat brain using 29350-1-AP

    IHC staining of rat brain using 29350-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29350-1-AP (ABR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ABR Polyclonal antibody

ABR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29350-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

active BCR related gene, MDB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U-251 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29350-1-AP targets ABR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29522

Product name: Recombinant human ABR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-87 aa of NM_021962

Sequence: MEPLSHRGLPRLSWIDTLYSNFSYGTDEYDGEGNEEQKGPPEGSETMPYIDESPTMSPQLSARSQGGGDGVSPTPPEGLAPGVEAGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 active BCR-related gene
别名 active BCR related gene, MDB
计算分子量 98 kDa
观测分子量97-100 kDa
GenBank蛋白编号NM_021962
基因名称 ABR
Gene ID (NCBI) 29
RRIDAB_3086122
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12979
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABR (Active breakpoint cluster region-related protein) is the only protein known in humans and mice to share high homology with BCR (68% amino acid identity). BCR(Breakpoint cluster region) gene was originally identified due to its involvement in a specific chromosomal translocation that causes the development of chronic myeloid leukemia and a subset of acute lymphoblastic leukemia. The C-terminus is a GTPase-activating protein domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. (PMID: 17116687, PMID: 37507586)

实验方案

Product Specific Protocols
WB protocol for ABR antibody 29350-1-APDownload protocol
IHC protocol for ABR antibody 29350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols