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验证数据展示

  • IHC staining of human brain using 20494-1-AP

    IHC staining of human brain using 20494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 20494-1-AP

    IHC staining of human brain using 20494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 20494-1-AP

    WB analysis using 20494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20494-1-AP (ABHD6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20494-1-AP

    IHC staining of mouse brain using 20494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of C6 using 20494-1-AP

    IF Staining of C6 using 20494-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using ABHD6 antibody (20494-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of RAW 264.7 using 20494-1-AP

    IF Staining of RAW 264.7 using 20494-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using ABHD6 antibody (20494-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

ABHD6 Polyclonal antibody

ABHD6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20494-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

2-arachidonoylglycerol hydrolase, ABHD 6, Abhydrolase domain-containing protein 6, EC:3.1.1.23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat spleen tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC6 cells, RAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20494-1-AP targets ABHD6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 ABHD6 fusion protein Ag13968 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 6
别名 2-arachidonoylglycerol hydrolase, ABHD 6, Abhydrolase domain-containing protein 6, EC:3.1.1.23
计算分子量 337 aa, 38 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC001698
基因名称 ABHD6
Gene ID (NCBI) 57406
RRIDAB_2878694
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV23
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

α/β-hydrolase domain 6 (ABHD6) is a lipase linked to physiological functions affecting energy metabolism. ABHD6, which was first identified in the brain ( PMID: 18096503) , is a ubiquitously expressed lipase.

实验方案

Product Specific Protocols
WB protocol for ABHD6 antibody 20494-1-APDownload protocol
IHC protocol for ABHD6 antibody 20494-1-APDownload protocol
IF protocol for ABHD6 antibody 20494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neuron

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.

Authors - Jochen Schwenk
View Article
mouseWB

Pharmacol Res

Wwl70-induced ABHD6 inhibition attenuates memory deficits and pathological phenotypes in APPswe/PS1dE9 mice

Authors - Zhiwei Xue
View Article
WB

Front Immunol

RGMa Participates in the Blood-Brain Barrier Dysfunction Through BMP/BMPR/YAP Signaling in Multiple Sclerosis.

Authors - Lei Zhang
View Article
humanWB

Mol Carcinog

ABHD6 suppresses colorectal cancer progression via AKT signaling pathway

Authors - Xiaoyu Xiong
  • KD Validated
View Article