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验证数据展示

  • FC experiment of mouse splenocytes using PE-12201

    FC experiment of mouse splenocytes using PE-12201

    1x10^6 mouse splenocytes were intracellularly stained with 0.1 ug PE-conjugated ABHD5 Polyclonal antibody (PE-12201)(red), or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of mouse thymocytes using PE-12201

    FC experiment of mouse thymocytes using PE-12201

    1x10^6 mouse thymocytes were intracellularly stained with 0.1 ug PE-conjugated ABHD5 Polyclonal antibody (PE-12201)(red), or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

PE-conjugated ABHD5 Polyclonal antibody

ABHD5 Polyclonal Antibody for ELISA
Cat No. PE-12201

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

ELISA

别名

1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, CDS, CGI58, EC:2.3.1.51

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inmouse splenocytes, mouse thymocytes
Planning an IHC experiment? We recommend our IHCeasy ABHD5 Ready-To-Use IHC Kit. ABHD5 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.1 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-12201 targets ABHD5 in ELISA applications and shows reactivity with human, mouse samples.

经测试应用 ELISA Application Description
经测试反应性 human, mouse
免疫原 ABHD5 fusion protein Ag2840 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 5
别名 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, CDS, CGI58, EC:2.3.1.51
计算分子量 349 aa, 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC021958
基因名称 ABHD5
Gene ID (NCBI) 51099
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WTS1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

实验方案

Product Specific Protocols
FC protocol for PE ABHD5 antibody PE-12201Download protocol
Standard Protocols
Click here to view our Standard Protocols