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验证数据展示

  • WB analysis of U-937 using 14269-1-AP

    WB analysis of U-937 using 14269-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14269-1-AP (ABCG4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14269-1-AP

    IHC staining of human liver cancer using 14269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14269-1-AP (ABCG4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 14269-1-AP

    IHC staining of human liver cancer using 14269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14269-1-AP (ABCG4 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse eye using 14269-1-AP

    IHC staining of mouse eye using 14269-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 14269-1-AP (ABCG4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ABCG4 Polyclonal antibody

ABCG4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14269-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ATP-binding cassette sub-family G member 4, EC:7.6.2.-, WHITE2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-937 cells, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue, mouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14269-1-AP targets ABCG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag5587

Product name: Recombinant human ABCG4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-356 aa of BC041091

Sequence: MAEKALEAVGCGLGPGAVAMAVTLEDGAEPPVLTTHLKKVENHITEAQRFSHLPKRSAVDIEFVELSYSVREGPCWRKRGYKTLLKCLSGKFCRRELIGIMGPSGAGKSTFMNILAGYRESGMKGQILVNGRPRELRTFRKMSCYIMQDDMLLPHLTVLEAMMVSANLKLSEKQEVKKELVTEILTALGLMSCSHTRTALLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKSLAQGGRTIICTIHQPSAKLFEMFDKLYILSQGQCIFKGVVTNLIPYLKGLGLHCPTYHNPADFIIEVASGEYGDLNPMLFRAVQNGLCAMAEKKSSPEKNEVPAPCPPCPP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family G (WHITE), member 4
别名 ATP-binding cassette sub-family G member 4, EC:7.6.2.-, WHITE2
计算分子量 72 kDa
观测分子量 60 kDa, 90-100 kDa
GenBank蛋白编号BC041091
基因名称 ABCG4
Gene ID (NCBI) 64137
RRIDAB_2220436
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H172
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCG4 antibody 14269-1-APDownload protocol
IHC protocol for ABCG4 antibody 14269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

PLoS One

High ABCG4 Expression Is Associated with Poor Prognosis in Non-Small-Cell Lung Cancer Patients Treated with Cisplatin-Based Chemotherapy.

Authors - Guang Yang
View Article