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验证数据展示

  • WB analysis using 13950-1-AP (same clone as 13950-1-PBS)

    WB analysis using 13950-1-AP (same clone as 13950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 13950-1-AP (same clone as 13950-1-PBS)

    IP experiment of K-562 using 13950-1-AP (same clone as 13950-1-PBS)

    IP result of anti-ABCF1 (IP:13950-1-AP, 4ug; Detection:13950-1-AP 1:6000) with K-562 cells lysate 1360 ug. This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13950-1-AP (same clone as 13950-1-PBS)

    IF Staining of HeLa using 13950-1-AP (same clone as 13950-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCF1 antibody (13950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 13950-1-AP (same clone as 13950-1-PBS)

    IHC staining of human stomach cancer using 13950-1-AP (same clone as 13950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 13950-1-AP (same clone as 13950-1-PBS)

    IHC staining of human stomach cancer using 13950-1-AP (same clone as 13950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ABCF1 Polyclonal antibody, PBS Only

ABCF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13950-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

TNF-alpha-stimulated ABC protein, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, ATP binding cassette 50, ABCF 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13950-1-PBS targets ABCF1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5061

Product name: Recombinant human ABCF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 496-845 aa of BC034488

Sequence: GWRKTLLIVSHDQGFLDDVCTDIIHLDAQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEALTRKQQKCRRKNQDEESQEAPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGVTFGYQGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTHGEMRKNHRLKIGFFNQQYAEQLRMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFEDYKREVLEALGEVMVSRPRE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family F (GCN20), member 1
别名 TNF-alpha-stimulated ABC protein, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, ATP binding cassette 50, ABCF 1
计算分子量 92 kDa
观测分子量 110 kDa
GenBank蛋白编号BC034488
基因名称 ABCF1
Gene ID (NCBI) 23
RRIDAB_2242044
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NE71
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABCF1, also known as ABC27, and ABC50, is a member of the superfamily of ATP-binding cassette (ABC) transporters and contains two ABC transporter domains. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, ABCF1 lacks the transmembrane domains that are characteristic of most ABC transporters. ABCF1 may be regulated by tumor necrosis factor-alpha and play a role in the enhancement of protein synthesis and thus participate in the inflammatory processes(PMID: 9790762).

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