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验证数据展示

  • WB analysis of mouse liver using 87047-1-RR (same clone as 87047-1-PBS)

    WB analysis of mouse liver using 87047-1-RR (same clone as 87047-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 87047-1-RR (ABCD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87047-1-PBS in a different storage buffer formulation.

  • WB analysis using 87047-1-RR (same clone as 87047-1-PBS)

    WB analysis using 87047-1-RR (same clone as 87047-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87047-1-RR (ABCD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87047-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 87047-1-RR (same clone as 87047-1-PBS)

    WB analysis of HEK-293 using 87047-1-RR (same clone as 87047-1-PBS)

    WB result of ABCD3 antibody (87047-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCD3 transfected HEK-293 cells. This data was developed using the same antibody clone with 87047-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 87047-1-RR (same clone as 87047-1-PBS)

    IF Staining of PC-3 using 87047-1-RR (same clone as 87047-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using ABCD3 antibody (87047-1-RR, Clone: 252233H1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87047-1-PBS in a different storage buffer formulation.

ABCD3 Recombinant monoclonal antibody, PBS Only

ABCD3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87047-1-PBS

产品说明书

CloneNo. 252233H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

ATP-binding cassette sub-family D member 3, EC:3.1.2.-, PMP70, PXMP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ABCD3 Ready-To-Use IHC Kit. ABCD3 primary antibody included.

产品信息

87047-1-PBS targets ABCD3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag24760

Product name: Recombinant human ABCD3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-124 aa of BC068509

Sequence: MAAFSKYLTARNSSLAGAAFLLLCLLHKRRRALGLHGKKSGKPPLQNNEKEGKKERAVVDKVFFSRLIQILKIMVPRTFCKETGYLVLIAVMLVSRTYCDVWMIQNGTLIESGIIGRSRKDFKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP-binding cassette, sub-family D (ALD), member 3
别名 ATP-binding cassette sub-family D member 3, EC:3.1.2.-, PMP70, PXMP1
观测分子量 70 kDa
GenBank蛋白编号BC068509
基因名称 ABCD3
Gene ID (NCBI) 5825
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP28288
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABCD3 belongs to the superfamily of ATP-binding cassette (ABC) transporters and is a peroxisomal membrane transporter involved in the transport of branched-chain fatty acids and C27 bile acids into the peroxisome. ABCD3 plays a role in the regulation of LCFAs and energy metabolism, namely in the degradation and biosynthesis of fatty acids via beta-oxidation (PMID: 24333844). Mutations in ABCD3 are associated with congenital bile acid synthesis defect 5 (CBAS5)(PMID: 25168382).

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